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MV1469 Datasheet

HiFluoro Pseudomonas HiVeg Agar Base Technical Data

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HiFluoro Pseudomonas HiVeg Agar Base
MV1469
HiFluoro Pseudomonas HiVeg Agar Base is recommended for selective isolation of Pseudomonas aeruginosa from
clinical and non-clinical specimens by fluorogenic method.
Composition**
Ingredients
HiVeg Peptone No.2
Magnesium chloride
Potassium sulphate
Cetrimide
Fluorogenic mixture
Agar
Final pH ( at 25°C)
**Formula adjusted, standardized to suit performance parameters
Gms / Litre
18.000
1.400
10.000
0.300
2.050
15.000
7.2±0.2
Directions
Suspend 46.75 grams in 1000 ml distilled water containing 10ml glycerol. Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Pseudomonas aeruginosa (also known as Pseudomonas pyocyanea ) is a gram-negative, aerobic, rod-shaped bacterium.
Like other Pseudomonads, P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein
(yellow-green and fluorescent), and pyorubin (red-brown). King et al developed Pseudomonas Agar P (i.e. King A media)
for enhancing pyocyanin and pyorubin production and Pseudomonas Agar F (i.e. King B media) for enhancing fluorescein
production (1). HiFluoro Pseudomonas HiVeg Agar Base is prepared by completely replacing animal based peptones with veg
peptones. This media is a modification of HiFluoro Pseudomonas Agar Base which is devised based on the formula described
by King et al. (1) except fluorogenic mixture. It is used as the selective medium for the isolation of P. aeruginosa from
pus, sputum and drains etc.
Cetrimide (Cetyltrimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than P. aeruginosa
. It acts as a quaternary ammonium compound, cationic detergent that causes nitrogen and phosphorus to be released from
bacterial cells other than P.aeruginosa . P.aeruginosa cleaves the fluorogenic compound to release the fluorogen which
produces a visible fluorescence under long wave UV light.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber colourec opalescent gel forms in Petri plates
Reaction
Reaction of 4.675 % w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
Please refer disclaimer Overleaf.
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MV1469 Datasheet

HiFluoro Pseudomonas HiVeg Agar Base Technical Data

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HiMedia Laboratories
Technical Data
Organism
Cultural Response
Pseudomonas aeruginosa
ATCC 27853
Stenotrophomonas
maltophila ATCC 13637
Staphylococcus aureus
ATCC 25923
Escherichia coli ATCC
25922
Inoculum
(CFU)
50-100
>=10³
>=10³
>=10³
Growth
luxuriant
inhibited
inhibited
inhibited
Recovery
>=50%
0%
0%
0%
Fluorescence
(under uv)
positive
Storage and Shelf Life
Store dehydrated and prepared medium at 2-8°C in tightly closed container. Use before expiry period on the label.
Reference
1.King, Ward and Raney, 1954, J. Lab. Clin. Med., 44:301.
Revision : 1 / 2011
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this
and other related HiMedia™ publications. The information contained in this publication is based on our research and development work
and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications
and information related to the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for
laboratory, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered
as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147
1919 Email: techhelp@himedialabs.com
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Part Number MV1469
Description HiFluoro Pseudomonas HiVeg Agar Base Technical Data
Maker Himedia
Total Page 2 Pages
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