SC-31155 Datasheet | Santa Cruz Biotechnology

SANTA CRUZ BIOTECHNOLOGY, INC. .. EGFR (N-20): sc-31155 The Power to Question BACKGROUND The EGF receptor family prises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (Erb B-2, HER2), Erb B-3 (HER3) and Erb B-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Exons in the EGFR gene product are frequently either deleted or duplicated to produce deletion mutants (DM) or tandem duplication mutants (TDM), respectively, which are detected at various molecular weights. EGFR binds several ligands, including epidermal growth factor (EGF), transforming growth factor α (TGFα), Amphiregulin and heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target. APPLICATIONS EGFR (N-20) is remended for detection of EGF receptor of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1- 2 µg per 100- 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). Suitable for use as control antibody for EGFR si RNA (h): sc-29301 and EGFR si RNA (m): sc-29302. Molecular Weight of EGFR: 170 k Da. Positive Controls: human breast carcinoma tissue, SK-BR-3 cell lysate: sc-2218 or A-431 whole cell lysate:...