SC-32750 Datasheet | Santa Cruz Biotechnology

SANTA CRUZ BIOTECHNOLOGY, INC. .. CD223 (C9B7W): sc-32750 The Power to Question BACKGROUND CD223 (Lymphocyte activation gene-3, LAG-3) is a high affinity MHC class II ligand present on the surface of CD4+/CD8+ T cells and NK cells. CD223 shares homology in structure to CD4 molecules, having four similar extracellular Ig-like domains and structural motifs between D1-D3 and D2-D4 domains. CD223 has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. CD223+/CD4+/CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. CD223 inhibits CD4-dependent T cell function via its cytoplasmic domain . CD223 Lys-468 within a conserved "KIEELE" motif is essential for interaction with downstream signaling molecules. APPLICATIONS CD223 (C9B7W) is remended for detection of CD223 of mouse origin by flow cytometry (1 µg per 1 x 106 cells). Suitable for use as control antibody for CD223 si RNA (m): sc-44548. Molecular Weight of CD223: 70 k Da. DATA REFERENCES 1. Demeure, C.E., et al. 2001. T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG3): role of LAG-3/MHC class II interactions in cell-cell contacts. Eur. J. Cancer 37: 1709-1718. 2. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins University, Baltimore, MD. MIM Number: 153337. World Wide Web URL: http://.ncbi.nlm.nih.gov/omim/ 3. Locus Link Report (Locus ID: 3902). http://.ncbi.nlm.nih.gov/Locus Link/ 4. Andreae, S., et al. 2002. Maturation and activation of dendritic cells induced by lymphocyte activation gene-3 (CD223). J. Immunol. 168: 3874-3880. 5. Workman, C.J., et al. 2002. Phenotypic analysis of the murine CD4-related glycoprotein, CD223 (LAG-3). Eur. J. Immunol. 32: 2255-2263. 6. Workman, C.J., et al. 2002. Cutting edge: molecular analysis of the...