SC-375 Datasheet | Santa Cruz Biotechnology

SANTA CRUZ BIOTECHNOLOGY, INC. .. Gγ 3 (K-20): sc-375 The Power to Question BACKGROUND Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the βγ subunits. It is being increasingly clear that different G protein plexes expressed in different tissues carry structurally distinct members of the γ, as well as the α and β, subunits and that preferential associations between members of subunit families increase G protein functional diversity. APPLICATIONS Gγ 3 (K-20) is remended for detection of Gγ 3 of broad origin by Western blotting, immunoprecipitation [1- 2 µg per 100- 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). Suitable for use as control antibody for Gγ 3 si RNA (h): sc-41776 and Gγ 3 si RNA (m): sc-41777. Molecular Weight of Gγ 3: 3-7 k Da. Positive Controls: mouse cerebellum extract: sc-2403 or rat brain extract: sc-2392. DATA 34 K - 46 K 30 K 22 K 14 K 7K3K23 K - REFERENCES 1. Gautam, N., et al. 1990. G protein diversity is increased by associations with a variety of γ subunits. Proc. Natl. Acad. Sci. USA 87: 7973-7977. 2. Simon, M.I., et al. 1991. Diversity of G proteins in signal transduction. Science 252: 802-808....